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Lethally irradiated rats were injected with a conjugate of 4,4′-bis[2-amino-2-methyl-1-aziridinyl)ethoxy]maleimide (BMD), an aryl halide derivative, with keyhole limpet hemocyanin (KLH) or ovalbumin (OA). They developed specific tolerance to KLH but not to OA, with the tolerogenic effect being operative even when animals were sensitised by prior injection of BMD-KLH. In the latter case, however, pre-treatment of animals with OA produced the state of hapten allergy analogous to that found following immunisation with the same antigen. Thus, tolerance was operative only in the presence of a pre-existing antigen-specific response. In vivo and in vitro experiments indicate that T cells are not the effectors of the tolerance; however, the possibility of suppression of

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